Ligation-anchored PCR: a simple amplification technique with single-sided specificity.
نویسندگان
چکیده
منابع مشابه
Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique
Mapping sites within the genome that are hypersensitive to digestion with DNaseI is an important method for identifying DNA elements that regulate transcription. The standard approach to locating these DNaseI-hypersensitive sites (DHSs) has been to use Southern blotting techniques, although we, and others, have recently published alternative methods using a range of technologies including high-...
متن کاملModal Characteristics of Composite Beams with Single Delamination- A Simple Analytical Technique
In the present research, the effects of delamination size and location on vibration characteristics of laminated composite beams are investigated both analytically and numerically. In the analytical method, the delaminated beam is modeled as four interconnected Euler–Bernoulli beams and the constrained and free mode models are both simulated. The differential stretching and the bending-extensio...
متن کاملA Nonspecific Primer Anchored PCR Technique for Chromosome Walking
A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperatures were varied to control the priming. Target sequences were obtained by PCR with random primer an...
متن کاملGene expression analysis in platelets from a single donor: evaluation of a PCR-based amplification technique.
BACKGROUND Genetic analysis of platelet mRNA may facilitate the diagnosis of disorders affecting the megakaryocytic-platelet lineage. Its use, however, is limited by the exceptionally small yield of platelet mRNA and the risk of leukocyte contamination during platelet preparation. METHODS We depleted platelet suspensions of leukocytes by filtration and used a PCR-based RNA amplification step ...
متن کاملSIMPLE LIGATION VERSUS STUMP INVAGINATION DURING APPENDICECTOMY-A PROSPECTIVE TRIAL
A prospective, randomized trial on 416 consecutive cases of appendicectomies was carried out in which half of the cases had simple ligation of the stump and the rest had invagination of the stump in addition. The two groups were matched for age, sex, state of personal hygiene and nutrition. The incidence of wound infection, and early and late post-operative complications remained comparabl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.20.9823